Invited Speaker Australian & New Zealand Society of Magnetic Resonance Conference 2017

The First q-space NMR Microscopy Experiment in the Callaghan Lab (#40)

Yang Xia 1
  1. Oakland University, Rochester, MICHIGAN, United States

I completed the MSc (1989) and PhD (1992) dissertation research under the guidance of Paul T Callaghan in the Physics and Biophysics Department of Massey University (Palmerston North, New Zealand). The topics of my dissertations were the physics of NMR microscopy (µMRI) and the development of quantitative µMRI for the studies of polymer dynamics and plant vascular flows. My projects were documented in 12 journal papers during my 6.5 years in his lab. This presentation recaps what were done in order to carry out the first successful experiment that simultaneously constructed both velocity and diffusion maps at high resolution, on a JEOL FX-60 NMR spectrometer which had a 1.4T electromagnet and a TI980A computer. The image acquisition and reconstruction utilized a Hitachi MB-16000 computer, which ran MS DOS 1.1, and had two 5.25” floppy drives and 256 kB memory. The smallest raw data in my intended experiments had the size of 311kB, which were more than the entire computer memory and an entire floppy disc. Although it looked like an impossible mission, the first experiment on flow and diffusion was completed successful in the afternoon of 1987 Sep 11. The experimental data was published together with the first introduction of q-space concept in NMR and MRI in 1988 (J Phys E: Sci Instrum 21, 820, 1988). With these home-built devices, I completed several challenging experiments in flow and diffusion imaging, including the first in vivo imaging of a live wheat grain, the measurement of in vivo flow as low as 10 µm/s, and the measurement of self-diffusion as low as 10-14 m2/s.

  1. 1. Callaghan PT, Eccles CD, Xia Y. 1988. NMR microscopy of dynamic displacements: k-space and q-space imaging. J Phys E: Sci Instrum 21:820-822. 2. Jenner CF, Xia Y, Eccles CD, et al. 1988. Circulation of water within wheat grain revealed by nuclear magnetic resonance micro-imaging. Nature 336:399. 3. Xia Y, Callaghan PT. 1991. Study of Shear Thinning in High Polymer Solution Using Dynamic NMR Microscopy. Macro Mol 24:4777-4786. 4. Xia Y, Jeffrey KR, Callaghan PT. 1992. Purpose-designed Probe for Dynamic NMR Microscopy in an Electromagnet. Magn Reson Imaging 10:411-426.